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新冠研究蛋白、抗体等试剂库
发布时间：2022-06-15 16:16:45 点击：2173
新冠研究蛋白、抗体等试剂库
由新冠病毒引起的新冠肺炎疫情影响了全球的医疗卫生系统以及经济，同时不断突变的新毒株对新冠疫苗、治疗药物以及诊断都带来了巨大的挑战。义翘神州一直密切关注新冠病毒的进化情况，已保证第一时间研发出新冠病毒相关的蛋白、抗体等试剂。目前，我们的蛋白、抗体试剂以广泛被全球科学家们用于新冠相关的各方面的研究，已助力正式发表900多篇科研论文。除此之外，我们的试剂产品也被用于免疫检测试剂的核心原料用于其开发上市。我们已开发了拥有200+新冠突变体蛋白的突变体蛋白库，覆盖了VOC, VOI等突变位点。这些突变体蛋白也已经被研究着们用于新冠病毒免疫逃逸等相关研究。
重组新冠抗原
重组Spike蛋白
重组N蛋白
重组突变体蛋白库
酶类，NSP蛋白等
宿主受体ACE2蛋白
SARS-CoV-2 Spike 蛋白推荐
| 货号 | 抗原 | 表达宿主 | 标签 |
| 40591-V08H | S1 | HEK293 Cells | His |
| 40590-V08B | S2 | Baculovirus-Insect Cells | His |
| 40592-V05H | RBD | HEK293 Cells | mFc |
| 40592-V08B | RBD | Baculovirus-Insect Cells | His |
| 40592-V08H | RBD | HEK293 Cells | His |
更多SARS-CoV-2 Spike 蛋白
| 货号 | 抗原 | 表达宿主 | 标签 |
| 40589-V08H4 | S1+S2 ECD | HEK293 Cells | His |
| 40591-V02H | S1 | HEK293 Cells | Fc |
| 40591-V05H1 | S1 | HEK293 Cells | mFc |
| 40591-V08B1 | S1 | Baculovirus-Insect Cells | His |
| 40591-V08H-B Biotinylated | S1 | HEK293 Cells | His |
| 40591-V27H-B Biotinylated | S1 | HEK293 Cells | AVI & His |
| 40591-V41H | S1 NTD | HEK293 Cells | Fc & AVI |
| 40591-V41H-B Biotinylated | S1 NTD | HEK293 Cells | Fc & AVI |
| 40591-V49H | S1 NTD | HEK293 Cells | His & AVI |
| 40591-V49H-B Biotinylated | S1 NTD | HEK293 Cells | His & AVI |
| 40589-V08B1 | S1+S2 ECD | Baculovirus-Insect Cells | His |
| 40589-V08B1-B Biotinylated | S1+S2 ECD | Baculovirus-Insect Cells | His |
| 40589-V27B-B Biotinylated | S1+S2 ECD | Baculovirus-Insect Cells | AVI & His |
| 40590-V02H | S2 | HEK293 Cells | Fc |
| 40590-V05B | S2 | Baculovirus-Insect Cells | mFc |
| 40590-V08B-B Biotinylated | S2 | Baculovirus-Insect Cells | His |
| 40590-V08H1 | S2 | HEK293 Cells | His |
| 40592-V02H | RBD | HEK293 Cells | Fc |
| 40592-V08B-B Biotinylated | RBD | Baculovirus-Insect Cells | His |
| 40592-V08H-B Biotinylated | RBD | HEK293 Cells | His |
| 40592-V27B-B Biotinylated | RBD | Baculovirus-Insect Cells | AVI & His |
| 40592-V27H-B Biotinylated | RBD | HEK293 Cells | AVI & His |
| 40592-V31H | RBD | HEK293 Cells | rFc |
| 40592-VNAH | RBD | HEK293 Cells |
新冠抗体试剂
义翘神州开发生产了针对新冠病毒不同抗原抗体试剂，特异性、中和效应以及多种应用均经过验证。
高亲和力
Binding affinity has been validated by BLI assay.
BLI: KD=0.006 nM
Anti-Spike Rabbit MAb (cat#:40592-R001)
BLI: KD=0.01 nM
Anti-N Rabbit MAb (cat#:40143-R040)
高特异性
ELISA assay has been taken to validate the cross-reactivity with Spike antigens from different Human Coronaviruses.
No cross-reactivity with SARS-CoV, MERS-CoV, HKU1, 229E, NL63, and OC43.
中和效应
The anti-RBD neutralizing antibody could effectively block the binding between recombinant spike RBD and ACE2 protein, and inhibit the infection of spike pseudovirus to ACE2 overexpressed HEK293T cells.
Anti-Spike RBD Mouse MAb (cat#: 40592-MM57)
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试剂盒采用双抗夹心ELISA法，可以快速定量检测N蛋白和S蛋白。试剂盒中使用的抗体均为义翘神州自主研发生产的单克隆抗体，已实现大量生产且可保证批次间稳定性。其中，S试剂盒经过重组蛋白和假病毒检测双重验证。 | SARS-CoV-2 N Detection Kit (KIT40588)  | |
| 更多详情：新冠病毒抗原蛋白检测试剂盒 | ||
| • 新冠病毒抑制剂筛选试剂盒 • 新冠病毒抗体滴度检测试剂盒 | ||
新冠试剂发表文献
截止到4月30日，已有近500篇正式发表的文章以及120篇预发表文章引用了义翘神州开发的新冠相关科研试剂。
| 题目 | 期刊 | 作者 | 年份 |
| Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity | Cell | Thomson, E,et al. | 2021 |
| Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection | Cell | Brouwer, PJM,et al. | 2021 |
| The antigenic anatomy of SARS-CoV-2 receptor binding domain | Cell | Dejnirattisai, W,et al. | 2021 |
| BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection | Cell | Mills, R,et al. | 2021 |
| COVID-19 immune features revealed by a large-scale single cell transcriptome atlas | Cell | Ren, X,et al. | 2021 |
| Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia | Cell | Asarnow, D,et al. | 2021 |
| Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients\' B cells | Cell | Cao, Y,et al. | 2020 |
| Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies | Cell | Wrapp, Daniel,et al. | 2020 |
| Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment | Cell | Sun, J,et al. | 2020 |
| Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2 | Cell | Wang, H,et al. | 2020 |
| A universal design of betacoronavirus vaccines against COVID-19, MERS and SARS | Cell | Dai, L,et al. | 2020 |
| The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity | Cell | Li, Q,et al. | 2020 |
| A thermostable mRNA vaccine against COVID-19 | Cell | Zhang, N,et al. | 2020 |
| High potency of a bivalent human VH domain in SARS-CoV-2 animal models | Cell | Li, W,et al. | 2020 |
| Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology | Cell | Piccoli, L,et al. | 2020 |
| Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant | Cell | Yurkovetskiy, L,et al. | 2020 |
| Structurally Resolved SARS-CoV-2 Antibody Shows High Efficacy in Severely Infected Hamsters and Provides a Potent Cocktail Pairing Strategy | Cell | Du, S,et al. | 2020 |
| Imbalance of regulatory and cytotoxic SARS-CoV-2-reactive CD4+ T cells in COVID-19 | Cell | Meckiff, B,et al. | 2020 |
| Quick COVID-19 Healers Sustain Anti-SARS-CoV-2 Antibody Production | Cell | Chen, Y,et al. | 2020 |
| Compromised humoral functional evolution tracks with SARS-CoV-2 mortality | Cell | Zohar, T,et al. | 2020 |
| A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2 | Cell | Hassan, AO,et al. | 2020 |
| Compromised SARS-CoV-2-specific placental antibody transfer | Cell | Atyeo, C,et al. | 2020 |
2019新型冠状病毒，即"SARS-CoV-2 (2019-nCoV)"，因2019年武汉病毒性肺炎病例而被发现，和2003年的 SARS冠状病毒以及2012年的MERS冠状病毒同属于冠状病毒科β属。
冠状病毒基因组依次编码棘突蛋白(spike protein)、包膜蛋白(Envelope protein)、膜蛋白(Membrane protein)和核衣壳蛋白(Nucleocapsid)。
棘突蛋白(spike protein) 是冠状病毒最重要的表面膜蛋白，含有两个亚基（subunit）， S1和S2。其中S1主要包含有受体结合区（receptorbinding domain， RBD），负责识别细胞的受体。S2含有膜融合过程所需的基本元件。
Spike 蛋白承担病毒与宿主细胞膜受体结合及膜融合功能，是宿主中和抗体的重要作用位点以及疫苗设计的关键靶点。SARS-CoV-2 (2019-nCoV) 的Spike蛋白与人ACE2互作来感染人的呼吸道上皮细胞。
核衣壳蛋白是冠状病毒中含量最丰富的蛋白。在病毒体组装过程中，N蛋白与病毒RNA结合并导致螺旋核衣壳的形成。核衣壳蛋白是一种高度免疫原性的磷蛋白，与病毒基因组复制和调节细胞信号通路有关。由于N蛋白的序列保守性和强大的免疫原性，N蛋白常被作为冠状病毒诊断检测工具
义翘神州于1月22日全球首发新冠病毒（SARS-CoV-2）重要靶点Spike RBD重组蛋白。为了更好的支持新冠肺炎的研究，义翘神州开发生产了数百种新冠病毒（SARS-CoV-2）相关科研试剂，包括抗原蛋白（N蛋白、Spike全长胞外域、 Spike S1、Spike S2、Spike RBD, Plpro, MTase, NSP3, NSP8，NSP9）、单抗、抗原蛋白检测试剂盒、抑制剂筛选试剂盒、抗体滴度检测试剂盒以及即用型表达基因。这些科研试剂已被全球数百家研究机构用于疫苗和治疗性抗体开发。义翘神州还可以提供新冠假病毒中和检测服务，可用于筛选出具有中和活性的抗体或血清，支持药物研发在细胞水平上的评估。
SARS-CoV-2 (2019-nCoV)抗原试剂产品列表
引用文献
• Xintian Xu.Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission.SCIENCE CHINA Life Sciences.2020
• Bo Diao, Zeqing Feng.Human Kidney is a Target for Novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection.medRxiv preprint
